Augmentation of Ca2+ signaling in astrocytic endfeet in the latent phase of temporal lobe epilepsy
نویسندگان
چکیده
Astrocytic endfeet are specialized cell compartments whose important homeostatic roles depend on their enrichment of water and ion channels anchored by the dystrophin associated protein complex (DAPC). This protein complex is known to disassemble in patients with mesial temporal lobe epilepsy and in the latent phase of experimental epilepsies. The mechanistic underpinning of this disassembly is an obvious target of future therapies, but remains unresolved. Here we show in a kainate model of temporal lobe epilepsy that astrocytic endfeet display an enhanced stimulation-evoked Ca(2+) signal that outlast the Ca(2+) signal in the cell bodies. While the amplitude of this Ca(2+) signal is reduced following group I/II metabotropic receptor (mGluR) blockade, the duration is sustained. Based on previous studies it has been hypothesized that the molecular disassembly in astrocytic endfeet is caused by dystrophin cleavage mediated by Ca(2+) dependent proteases. Using a newly developed genetically encoded Ca(2+) sensor, the present study bolsters this hypothesis by demonstrating long-lasting, enhanced stimulation-evoked Ca(2+) signals in astrocytic endfeet.
منابع مشابه
P119: Animal Models of Epilepsy: The Impact of some Chemoconvalsants on Animal Models
We summarize some of the most frequenthly used rodent animal models of temporal lobe epileps and the impact of chemoconvulsants on them. Temporal lobe epilepsy is the most common epilepsy in humans in which seizures spread to the neighboring cortiase and hippocampal neuron loss and other neuropathological take place. Temporal lobe epilepsy and the other form of epilepsy cannot acquired in chini...
متن کاملP-42: Protective Effects of Melatonin on The Testis in Post-Status Epilepticus Rats Following Lithium-Pilocarpin Injection As A Model of Temporal Lobe Epilepsy (TLE)
Background: Reproductive dysfunction and endocrine disorders are common among men with complex partial seizures of temporal lobe origin. More than 90% of men with epilepsy have abnormal semen analysis, including decreased sperm count, abnormal morphology, and impaired motility. The aim of this study was the assay of chronic treatment with melatonin during the latent phase and chronic phase of e...
متن کاملP-54: Melatonin Treatment Along Latent and Chronic Phases of Temporal Lobe Epilepsy, Protects Testes Against Destroying Effect of Lithium-Pilocarpine Induced Epileptic Rats
Background: Because of the observed protective effect of melatonin against TLE and the putative role of melatonin to influence neuroendocrine-gonadal axis, we tried to find out whether initiating the melatonin treatment after induction of Status Epilepticus (SE) and all along the different phases of epileptogenesis process, might be able to protect the testes and sperm against devastating effec...
متن کاملP 90: The Role of miR-146a in Inflammatory Process of Mesial Temporal Lobe Epilepsy (MTLE): Implication for Therapy
Epilepsy is the third most common chronic brain disorder which is characterized by an enduring predisposition to generate seizures. Mesial Temporal Lobe Epilepsy (MTLE) is the most common type of refractory epilepsy. Increasing evidence indicates that neuroinflammation plays a critical role in the pathogenesis of MTLE. Hundreds of micro-RNAs have been found to be abnormally expressed in epilept...
متن کاملIctal and Interictal Electroencephalography of Mesial and Lateral Temporal Lobe Epilepsy; A Comparative Study
Background: Epilepsy is considered as one of the most important disorders in neurology. Temporal lobe epilepsy is a form of epilepsy including two main types of mesial and lateral (neocortex). Objectives: Determination and comparison of electroencephalogram (EEG) pattern in the ictal and interictal phases of mesial and lateral temporal lobe epilepsy. Materials and Methods: This cross-sectiona...
متن کامل